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Typhoid IgG/IgM Rapid Test Cassette (Serum / Plasma)

Categories Infectious Disease Rapid Tests
Brand Name: SPAN
Model Number: RA0603I
Place of Origin: China
MOQ: 100 tests
Price: Negotiable
Payment Terms: T/T, Western Union, MoneyGram
Supply Ability: 1000000 pcs per month
Packaging Details: 40tests/box
Package: 40tests/box
Specificity: 100%
Sensitivity: 100%
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    Typhoid IgG/IgM Rapid Test Cassette (Serum / Plasma)


    Typhoid IgG/IgM Rapid Test Cassette (Serum / Plasma)

    Cat. No.: RA0603I

    Intended Use


    The Typhoid IgG/IgM Rapid Test is a lateral flow immunoassay for the simultaneous detection and differentiation of anti-Salmonella typhi (S. typhi)G and IgM in human serum or plasma. It is intended to be used as a screening test and as an aid in the diagnosis of infection with S. typhi. Any reactive specimen with the Typhoid IgG/IgM 2.0 Rapid Test must be confirmed with alternative testing method(s).

    Summary and Explanation of the Test


    Typhoid fever is caused by S. typhi, a Gram-negative bacterium. World-wide an estimated 17 million cases and 600,000 associated deaths occur annually1. Patients who are infected with HIV are at significantly increased risk of clinical infection with S. typhi2. Evidence of H. pylori infection also presents an increase risk of acquiring typhoid fever. 1-5% of patients become chronic carrier harboring S. typhi in the gallbladder.


    The clinical diagnosis of typhoid fever depends on the isolation of S. typhi from blood, bone marrow or a specific anatomic lesion. In the facilities that can not afford to perform this complicated and time-consuming procedure, Filix-Widal test is used to facilitate the diagnosis. However, many limitations lead to difficulties in the interpretation of the Widal test3,4.


    In contrast, the Typhoid IgG/IgM Rapid Test is a simple and rapid laboratory test. The test simultaneously detects and differentiates the IgG and the IgM antibodies to S. typhi specific antigen5 thus to aid in the determination of current or previous exposure to the S. typhi.


    Test Principle


    The Typhoid IgG/IgM Rapid Test is a lateral flow chromatographic immunoassay. The test cassette consists of: 1) a burgundy colored conjugate pad containing recombinant S. typhoid H antigen and O antigen conjugated with colloid gold (Typhoid conjugates) and rabbit IgG-gold conjugates, 2) a nitrocellulose membrane strip containing two test bands (M and G bands) and a control band (C band). The M band is pre-coated with monoclonal anti-human IgM for the detection of IgM anti-S. typhi, G band is pre-coated with reagents for the detection of IgG anti-S. typhi , and the C band is pre-coated with goat anti rabbit IgG.

    55


    When an adequate volume of test specimen is dispensed into the sample well of the cassette, the test specimen migrates by capillary action across the test cassette. Anti-S. typhi IgM if present in the patient specimen will bind to the Typhoid conjugates. The immunocomplex is then captured on the membrane by the pre-coated anti-human IgM antibody, forming a burgundy colored M band, indicating a S. typhi IgM positive test result.


    Anti-S. typhi IgG if present in the patient specimen will bind to the Typhoid conjugates. The immunocomplex is then captured by the pre-coated reagents on the membrane, forming a burgundy colored G band, indicating a S. typhi IgG positive test result.

    Absence of any test bands (M and G) suggests a negative result. The test contains an internal control (C band) which should exhibit a burgundy colored band of the immunocomplex of goat anti rabbit IgG/rabbit IgG-gold conjugate regardless of the color development on any of the test bands. Otherwise, the test result is invalid and the specimen must be retested with another device.

    Reagent and Materials Provided


    1. Each kit contains 25 or 30 test devices, each sealed in a foil pouch with three items inside:

    a. One cassette device.

    b. One plastic dropper.

    c. One desiccant.

    2. Sample Diluent (1 bottle, 5 mL)

    1. One package insert (instruction for use).

    Materials May Be required and not provide


    1. Positive Control (1 vial, red cap, 1 mL, Cat # R0160-P)
    2. Negative Control (1 vial, green cap, 1 mL, Cat # R0160-N)

    Materials required but not provided

    1. Clock or Timer

    Warnings and Precautions

    For in Vitro Diagnostic Use

    1. This package insert must be read completely before performing the test. Failure to follow the insert gives inaccurate test results.
    2. Do not open the sealed pouch, unless ready to conduct the assay.
    3. Do not use expired devices.
    4. Bring all reagents to room temperature (15°C-30°C) before use.
    5. Do not use the components in any other type of test kit as a substitute for the components in this kit.
    6. Do not use hemolized blood specimen for testing.
    7. Wear protective clothing and disposable gloves while handling the kit reagents and clinical specimens. Wash hands thoroughly after performing the test.
    8. Users of this test should follow the US CDC Universal Precautions for prevention of transmission of HIV, HBV and other blood-borne pathogens.
    9. Do not smoke, drink, or eat in areas where specimens or kit reagents are being handled.
    10. Dispose of all specimens and materials used to perform the test as biohazardous waste.
    11. Handle the Negative and Positive Control in the same manner as patient specimens.
    12. The testing results should be read within 15 minutes after a specimen is applied to the sample well or sample pad of the device. Read result after 15 minutes may give erroneous results.
    13. Do not perform the test in a room with strong air flow, ie. an electric fan or strong air-conditioning.

    Reagent Preparation and storage instructions


    All reagents are ready to use as supplied. Store unused test devices unopened at 2°C-30°C. The positive and negative controls should be kept at 2°C-8°C. If stored at 2°C-8°C, ensure that the test device is brought to room temperature before opening. The test device is stable through the expiration date printed on the sealed pouch. Do not freeze the kit or expose the kit over 30°C.


    Specimen Collection and Handling

    Consider any materials of human origin as infectious and handle them using standard biosafety procedures.


    Plasma

    1. Collect blood specimen into a lavender, blue or green top collection tube (containing EDTA, citrate or heparin, respectively in Vacutainer® ) by veinpuncture.

    2. Separate the plasma by centrifugation.

    3. Carefully withdraw the plasma into new pre-labeled tube.


    Serum

    1. Collect blood specimen into a red top collection tube (containing no anticoagulants in Vacutainer®) by veinpuncture.
    2. Allow the blood to clot.
    3. Separate the serum by centrifugation.
    4. Carefully withdraw the serum into a new pre-labeled tube.

    Test specimens as soon as possible after collecting. Store specimens at 2°C-8°C if not tested immediately.


    Store specimens at 2°C-8°C up to 5 days. The specimens should be frozen at -20°C for longer storage.


    Avoid multiple freeze-thaw cycles. Prior to testing, bring frozen specimens to room temperature slowly and mix gently. Specimens containing visible particulate matter should be clarified by centrifugation before testing. Do not use samples demonstrating gross lipemia, gross hemolysis or turbidity in order to avoid interference on result interpretation.


    Assay Procedure


    Step 1: Bring the specimen and test components to room temperature if refrigerated or frozen. Mix the specimen well prior to assay once thawed.

    Step 2: When ready to test, open the pouch at the notch and remove device. Place the test device on a clean, flat surface.

    Step 3: Be sure to label the device with specimen’s ID number.

    Step 4: Fill the pipette dropper with the specimen.

    Holding the dropper vertically, dispense 1 drop (about 30-45 mL) of specimen into the sample well making sure that there are no air bubbles.

    Then add 1 drop (about 35-50 µL) of Sample Diluent immediately.

    Onedropbuffer



    serumhand onedrop


    Results



    1 drop of specimen 1 drop of sample diluent 15 minutes


    Step 5: Set up timer.

    Step 6: Results can be read in 15 minutes. Positive results can be visible in as short as 1 minute.

    Don’t read result after 15 minutes. To avoid confusion, discard the test device after interpreting the result.


    Quality Control


    1. Internal Control:

    This test contains a built-in control feature, the C band. The C line develops after adding specimen and sample diluent. Otherwise, review the whole procedure and repeat test with a new device.


    1. External Control:

    Good Laboratory Practice recommends using the external controls, positive and negative, to assure the proper performing of the assay, in particularly, under the following circumstances:

    a. New operator uses the kit, prior to performing testing of specimens.

    1. A new lot of test kit is used.
    2. A new shipment of kits is used.
    3. The temperature used during storage of the kit fall outside of 2°C -30°C.
    4. The temperature of the test area falls outside of 15°C -30°C.

    Interpretation of Assay Result


    1. NEGATIVE OR NON-REACTIVE RESULT: If only the C band is present, the absence of any burgundy color in the both tesbands(M and G) indicates that no anti-S. typhi antibody is detected in the specimen. The result is negative or non-reactive.

    NEW-12

    2. POSITIVE OR REACTIVE RESULT:

    1. In addition to the presence of C band, if only M band is developed, the test indicates for the presence of anti- S. typhi IgM in the specimen. The result is IgM positive or reactive.

    NEW-5NEW-11

    1. In addition to the presence of C band, if only G band is developed, the test indicates for the presence of anti- S. typhi IgG in the specimen. The result is IgG positive or reactive.

    NEW-4NEW-9

    1. In addition to the presence of C band, both M and G bands are developed, the test indicates for the presence of anti-S. typhi IgG and IgM in the specimen. The result is both IgG and IgM positive or reactive.

    NEW-3
    NEW-10

    Samples with positive or reactive results should be confirmed with alternative testing method(s) and clinical findings before a positive determination is made.


    1. INVALID: If no C band is developed, the assay is invalid regardless of any burgundy color in the test bands as indicated below. Repeat the assay with a new device.

    NEW-7NEW-13NEW-8
    NEW-6

    PERFORMANCE CHARACTERISTICS


    1. Clinical Performance For IgM Test

    A total of 334 samples from susceptible subjects were tested by the Typhoid IgG/IgM2.0 Rapid Test and by a commercial S. typhi IgM EIA. Comparison for all subjects is showed in the following table.


    Typhoid IgG/IgM2.0 Rapid Test
    IgM EIAPositiveNegativeTotal
    Positive31334
    Negative2298300
    Total33302334

    Relative Sensitivity: 91% , Relative Specificity: 99.3%, Overall Agreement: 98.5%


    2. Clinical Performance For IgG Test

    A total of 314 samples from susceptible subjects were tested by the Typhoid IgG/IgM2.0 Rapid Test and by a commercial S. typhi IgG EIA kit. Comparison for all subjects is showed in the following table.


    Typhoid IgG/IgM2.0 Rapid Test
    IgG EIAPositiveNegativeTotal
    Positive13114
    Negative2298300
    Total15299314

    Relative Sensitivity: 92.9% , Relative Specificity: 99.3%, Overall Agreement: 99.0%


    Limitations of Test


    1. The Assay Procedure and the Test Result Interpretation must be followed closely when testing the presence of antibodies to S. typhi in serum or plasma from individual subjects. Failure to follow the procedure may give inaccurate results.
    2. The Typhoid IgG/IgM2.0 Rapid Test is limited to the qualitative detection of antibodies to S. typhi in human serum or plasma. The intensity of the test band does not have linear correlation with the antibody titer in the specimen.

    1. The Typhoid IgG/IgM2.0 Rapid Test also detects para-typhi antibodies.
    2. A negative result for an individual subject indicates absence of detectable anti-S. typhi antibodies. However, a negative test result does not preclude the possibility of exposure to S. typhi.
    3. A negative result can occur if the quantity of anti-S. typhi antibodies present in the specimen is below the detection limit of the assay, or the antibodies that are detected are not present during the stage of disease in which a sample is collected.
    4. If the symptom persists, while the result from Typhoid IgG/IgM2.0 Rapid Test is negative or non-reactive result, it is recommended to re-sample the patient few days late or test with an alternative test method, such as bacterial culture method.
    5. Some specimens containing unusually high titer of heterophile antibodies or rheumatoid factor may affect expected results.
    6. The results obtained with this test should only be interpreted in conjunction with other diagnostic procedures and clinical findings.

    References


    1. Ivanoff BN, Levine MM, Lambert PH. Vaccination against typhoid fever: present status. Bulletin of the World Health Organization 1994; 72: 957-71.

    2. Gotuzzo E, Frisancho O, Sanchez J, Liendo G, Carillo C, Black RE, Morris JG. Association between the acquired immunodeficiency syndrome and infection with Salmonella typhi or Salmonella paratyphi in an endemic typhoid area. Archives of Internal Medicine 1991; 151: 381-2.

    3. Clegg A, Passey M, Omena MK, et al. Re-evaluation of the Widal agglutination test in response to the changing pattern of typhoid fever in the highlands of Papua New Guinea. Acta Tropica 1994;57:255-63

    4. Pang T. False positive Widal test in nontyphoid Salmonella infection. Southeast Asian Journal of Tropical Medicine and Public Health 1989; 20: 163-4.

    5. Ismail A, Hai OK, Kader ZA. Demonstration of an antigenic protein specific for Salmonella typhi. Biochem Biophys Res Commun. 1991;181(1):301-5.


    Typhoid IgG/IgM Rapid Test Cassette (Serum / Plasma)

    Cat. No.: RA0603I

    Intended Use


    The Typhoid IgG/IgM Rapid Test is a lateral flow immunoassay for the simultaneous detection and differentiation of anti-Salmonella typhi (S. typhi)G and IgM in human serum or plasma. It is intended to be used as a screening test and as an aid in the diagnosis of infection with S. typhi. Any reactive specimen with the Typhoid IgG/IgM 2.0 Rapid Test must be confirmed with alternative testing method(s).

    Summary and Explanation of the Test


    Typhoid fever is caused by S. typhi, a Gram-negative bacterium. World-wide an estimated 17 million cases and 600,000 associated deaths occur annually1. Patients who are infected with HIV are at significantly increased risk of clinical infection with S. typhi2. Evidence of H. pylori infection also presents an increase risk of acquiring typhoid fever. 1-5% of patients become chronic carrier harboring S. typhi in the gallbladder.


    The clinical diagnosis of typhoid fever depends on the isolation of S. typhi from blood, bone marrow or a specific anatomic lesion. In the facilities that can not afford to perform this complicated and time-consuming procedure, Filix-Widal test is used to facilitate the diagnosis. However, many limitations lead to difficulties in the interpretation of the Widal test3,4.


    In contrast, the Typhoid IgG/IgM Rapid Test is a simple and rapid laboratory test. The test simultaneously detects and differentiates the IgG and the IgM antibodies to S. typhi specific antigen5 thus to aid in the determination of current or previous exposure to the S. typhi.


    Test Principle


    The Typhoid IgG/IgM Rapid Test is a lateral flow chromatographic immunoassay. The test cassette consists of: 1) a burgundy colored conjugate pad containing recombinant S. typhoid H antigen and O antigen conjugated with colloid gold (Typhoid conjugates) and rabbit IgG-gold conjugates, 2) a nitrocellulose membrane strip containing two test bands (M and G bands) and a control band (C band). The M band is pre-coated with monoclonal anti-human IgM for the detection of IgM anti-S. typhi, G band is pre-coated with reagents for the detection of IgG anti-S. typhi , and the C band is pre-coated with goat anti rabbit IgG.

    55


    When an adequate volume of test specimen is dispensed into the sample well of the cassette, the test specimen migrates by capillary action across the test cassette. Anti-S. typhi IgM if present in the patient specimen will bind to the Typhoid conjugates. The immunocomplex is then captured on the membrane by the pre-coated anti-human IgM antibody, forming a burgundy colored M band, indicating a S. typhi IgM positive test result.


    Anti-S. typhi IgG if present in the patient specimen will bind to the Typhoid conjugates. The immunocomplex is then captured by the pre-coated reagents on the membrane, forming a burgundy colored G band, indicating a S. typhi IgG positive test result.

    Absence of any test bands (M and G) suggests a negative result. The test contains an internal control (C band) which should exhibit a burgundy colored band of the immunocomplex of goat anti rabbit IgG/rabbit IgG-gold conjugate regardless of the color development on any of the test bands. Otherwise, the test result is invalid and the specimen must be retested with another device.

    Reagent and Materials Provided


    1. Each kit contains 25 or 30 test devices, each sealed in a foil pouch with three items inside:

    a. One cassette device.

    b. One plastic dropper.

    c. One desiccant.

    2. Sample Diluent (1 bottle, 5 mL)

    1. One package insert (instruction for use).

    Materials May Be required and not provide


    1. Positive Control (1 vial, red cap, 1 mL, Cat # R0160-P)
    2. Negative Control (1 vial, green cap, 1 mL, Cat # R0160-N)

    Materials required but not provided

    1. Clock or Timer

    Warnings and Precautions

    For in Vitro Diagnostic Use

    1. This package insert must be read completely before performing the test. Failure to follow the insert gives inaccurate test results.
    2. Do not open the sealed pouch, unless ready to conduct the assay.
    3. Do not use expired devices.
    4. Bring all reagents to room temperature (15°C-30°C) before use.
    5. Do not use the components in any other type of test kit as a substitute for the components in this kit.
    6. Do not use hemolized blood specimen for testing.
    7. Wear protective clothing and disposable gloves while handling the kit reagents and clinical specimens. Wash hands thoroughly after performing the test.
    8. Users of this test should follow the US CDC Universal Precautions for prevention of transmission of HIV, HBV and other blood-borne pathogens.
    9. Do not smoke, drink, or eat in areas where specimens or kit reagents are being handled.
    10. Dispose of all specimens and materials used to perform the test as biohazardous waste.
    11. Handle the Negative and Positive Control in the same manner as patient specimens.
    12. The testing results should be read within 15 minutes after a specimen is applied to the sample well or sample pad of the device. Read result after 15 minutes may give erroneous results.
    13. Do not perform the test in a room with strong air flow, ie. an electric fan or strong air-conditioning.

    Reagent Preparation and storage instructions


    All reagents are ready to use as supplied. Store unused test devices unopened at 2°C-30°C. The positive and negative controls should be kept at 2°C-8°C. If stored at 2°C-8°C, ensure that the test device is brought to room temperature before opening. The test device is stable through the expiration date printed on the sealed pouch. Do not freeze the kit or expose the kit over 30°C.


    Specimen Collection and Handling

    Consider any materials of human origin as infectious and handle them using standard biosafety procedures.


    Plasma

    1. Collect blood specimen into a lavender, blue or green top collection tube (containing EDTA, citrate or heparin, respectively in Vacutainer® ) by veinpuncture.

    2. Separate the plasma by centrifugation.

    3. Carefully withdraw the plasma into new pre-labeled tube.


    Serum

    1. Collect blood specimen into a red top collection tube (containing no anticoagulants in Vacutainer®) by veinpuncture.
    2. Allow the blood to clot.
    3. Separate the serum by centrifugation.
    4. Carefully withdraw the serum into a new pre-labeled tube.

    Test specimens as soon as possible after collecting. Store specimens at 2°C-8°C if not tested immediately.


    Store specimens at 2°C-8°C up to 5 days. The specimens should be frozen at -20°C for longer storage.


    Avoid multiple freeze-thaw cycles. Prior to testing, bring frozen specimens to room temperature slowly and mix gently. Specimens containing visible particulate matter should be clarified by centrifugation before testing. Do not use samples demonstrating gross lipemia, gross hemolysis or turbidity in order to avoid interference on result interpretation.


    Assay Procedure


    Step 1: Bring the specimen and test components to room temperature if refrigerated or frozen. Mix the specimen well prior to assay once thawed.

    Step 2: When ready to test, open the pouch at the notch and remove device. Place the test device on a clean, flat surface.

    Step 3: Be sure to label the device with specimen’s ID number.

    Step 4: Fill the pipette dropper with the specimen.

    Holding the dropper vertically, dispense 1 drop (about 30-45 mL) of specimen into the sample well making sure that there are no air bubbles.

    Then add 1 drop (about 35-50 µL) of Sample Diluent immediately.

    Onedropbuffer



    serumhand onedrop


    Results



    1 drop of specimen 1 drop of sample diluent 15 minutes


    Step 5: Set up timer.

    Step 6: Results can be read in 15 minutes. Positive results can be visible in as short as 1 minute.

    Don’t read result after 15 minutes. To avoid confusion, discard the test device after interpreting the result.


    Quality Control


    1. Internal Control:

    This test contains a built-in control feature, the C band. The C line develops after adding specimen and sample diluent. Otherwise, review the whole procedure and repeat test with a new device.


    1. External Control:

    Good Laboratory Practice recommends using the external controls, positive and negative, to assure the proper performing of the assay, in particularly, under the following circumstances:

    a. New operator uses the kit, prior to performing testing of specimens.

    1. A new lot of test kit is used.
    2. A new shipment of kits is used.
    3. The temperature used during storage of the kit fall outside of 2°C -30°C.
    4. The temperature of the test area falls outside of 15°C -30°C.

    Interpretation of Assay Result


    1. NEGATIVE OR NON-REACTIVE RESULT: If only the C band is present, the absence of any burgundy color in the both tesbands(M and G) indicates that no anti-S. typhi antibody is detected in the specimen. The result is negative or non-reactive.

    NEW-12

    2. POSITIVE OR REACTIVE RESULT:

    1. In addition to the presence of C band, if only M band is developed, the test indicates for the presence of anti- S. typhi IgM in the specimen. The result is IgM positive or reactive.

    NEW-5NEW-11

    1. In addition to the presence of C band, if only G band is developed, the test indicates for the presence of anti- S. typhi IgG in the specimen. The result is IgG positive or reactive.

    NEW-4NEW-9

    1. In addition to the presence of C band, both M and G bands are developed, the test indicates for the presence of anti-S. typhi IgG and IgM in the specimen. The result is both IgG and IgM positive or reactive.

    NEW-3
    NEW-10

    Samples with positive or reactive results should be confirmed with alternative testing method(s) and clinical findings before a positive determination is made.


    1. INVALID: If no C band is developed, the assay is invalid regardless of any burgundy color in the test bands as indicated below. Repeat the assay with a new device.

    NEW-7NEW-13NEW-8
    NEW-6

    PERFORMANCE CHARACTERISTICS


    1. Clinical Performance For IgM Test

    A total of 334 samples from susceptible subjects were tested by the Typhoid IgG/IgM2.0 Rapid Test and by a commercial S. typhi IgM EIA. Comparison for all subjects is showed in the following table.


    Typhoid IgG/IgM2.0 Rapid Test
    IgM EIAPositiveNegativeTotal
    Positive31334
    Negative2298300
    Total33302334

    Relative Sensitivity: 91% , Relative Specificity: 99.3%, Overall Agreement: 98.5%


    2. Clinical Performance For IgG Test

    A total of 314 samples from susceptible subjects were tested by the Typhoid IgG/IgM2.0 Rapid Test and by a commercial S. typhi IgG EIA kit. Comparison for all subjects is showed in the following table.


    Typhoid IgG/IgM2.0 Rapid Test
    IgG EIAPositiveNegativeTotal
    Positive13114
    Negative2298300
    Total15299314

    Relative Sensitivity: 92.9% , Relative Specificity: 99.3%, Overall Agreement: 99.0%


    Limitations of Test


    1. The Assay Procedure and the Test Result Interpretation must be followed closely when testing the presence of antibodies to S. typhi in serum or plasma from individual subjects. Failure to follow the procedure may give inaccurate results.
    2. The Typhoid IgG/IgM2.0 Rapid Test is limited to the qualitative detection of antibodies to S. typhi in human serum or plasma. The intensity of the test band does not have linear correlation with the antibody titer in the specimen.

    1. The Typhoid IgG/IgM2.0 Rapid Test also detects para-typhi antibodies.
    2. A negative result for an individual subject indicates absence of detectable anti-S. typhi antibodies. However, a negative test result does not preclude the possibility of exposure to S. typhi.
    3. A negative result can occur if the quantity of anti-S. typhi antibodies present in the specimen is below the detection limit of the assay, or the antibodies that are detected are not present during the stage of disease in which a sample is collected.
    4. If the symptom persists, while the result from Typhoid IgG/IgM2.0 Rapid Test is negative or non-reactive result, it is recommended to re-sample the patient few days late or test with an alternative test method, such as bacterial culture method.
    5. Some specimens containing unusually high titer of heterophile antibodies or rheumatoid factor may affect expected results.
    6. The results obtained with this test should only be interpreted in conjunction with other diagnostic procedures and clinical findings.

    References


    1. Ivanoff BN, Levine MM, Lambert PH. Vaccination against typhoid fever: present status. Bulletin of the World Health Organization 1994; 72: 957-71.

    2. Gotuzzo E, Frisancho O, Sanchez J, Liendo G, Carillo C, Black RE, Morris JG. Association between the acquired immunodeficiency syndrome and infection with Salmonella typhi or Salmonella paratyphi in an endemic typhoid area. Archives of Internal Medicine 1991; 151: 381-2.

    3. Clegg A, Passey M, Omena MK, et al. Re-evaluation of the Widal agglutination test in response to the changing pattern of typhoid fever in the highlands of Papua New Guinea. Acta Tropica 1994;57:255-63

    4. Pang T. False positive Widal test in nontyphoid Salmonella infection. Southeast Asian Journal of Tropical Medicine and Public Health 1989; 20: 163-4.

    5. Ismail A, Hai OK, Kader ZA. Demonstration of an antigenic protein specific for Salmonella typhi. Biochem Biophys Res Commun. 1991;181(1):301-5.


    Span Biotech Ltd. is a research based company for rapid tests, with strong support from National Key Laboratory of Technology Projects of 10th and 11th Five-Year Plan and Faculty of Life Sciences of HuBei University. SpanBio also housed a R&D team that is developing gene recombination, cell cultivation and protein purification techniques. SpanBio pays strict attention on rapid tests for human being, animal diseases and food safety detection. It provides a number of customized services to professional distributors and partnering affiliates with excellent quality, competitive prices and super service.


    Our mission:


    • Always best of all and always pay attention to innovation.
    • Special customized service tightly following customers’ requests.
    • Integrated excellent quality, competitive prices and super service together.

    Span Biotech Ltd.

    Add: 6A, Unit 5, Building 3, Sunshine Aloha, Bantian Industrial Park, Banxuegang Road , Longgang Shenzhen 518129

    Tel:+86-755-89589611

    E-mail: info@spanbio.com


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