β-lactamase ELISA Kit , competitive enzyme immunoassay , detect beta-lactam containing antibiotics

β-lactamase ELISA Kit

Purpose

This kit allows for the determination of β-lactamase activity in milk.

Principle of the assay

The kit assay β-lactamase level in the sample,use Purified β-lactamase antibody to coat microtiter plate wells, make solid-phase antibody, then add β-lactamase to wells, Combined β-lactamase antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of β-lactamase in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Materials provided with the kit

Reqired Materials Not Provided With the Kit

• Microtiter plate reader (450 nm)
• Incubator
• Tissue Mixer (e.g. Omni TissueMaster Homogenizer)
• Vortex mixer (e.g. Gneie Vortex mixer from VWR)
• 10, 20, 100 and 1000 mL pipettes
• Multi-channel pipette: 50-300 mL (Optional)
• n-Hexane
• Distilled deionized water
• Disposable plastic reservoirs
• Paper towels
• Centrifuge